Semen Analysis

Semen Analysis

Spermogram Department | Male Fertility Assessment

A spermogram (semen analysis) is a specialized laboratory test used to evaluate sperm quality and male reproductive health. This test plays a critical role in the assessment of male infertility and in planning fertility treatments. At Dr. Daneshbod Pathobiology Laboratory, spermogram testing is performed with high accuracy and in accordance with international standards.

The Spermogram Department of Dr. Daneshbod Laboratory provides specialized services for evaluating male fertility by adhering to international guidelines—particularly those of the World Health Organization (WHO)—and utilizing precise laboratory equipment. Semen samples are collected according to proper protocols, and key parameters such as semen volume, pH, sperm count, motility, morphology, as well as the

What Is a Spermogram?

A spermogram, also known as semen analysis, is a specialized test that evaluates both the quality and quantity of sperm. It assesses the physical, microscopic, and cellular characteristics of semen and helps identify potential causes of male infertility. Performing a spermogram is usually the first diagnostic step in the evaluation and treatment of infertility.

Parameters Evaluated in a Spermogram

The following key parameters are measured during semen analysis:

• Semen Volume: Normal range is 1.5 to 5 mL

• Sperm Concentration (Sperm Count): Number of sperm per milliliter

• Sperm Motility: Percentage and type of motile sperm

• Sperm Morphology: Percentage of sperm with normal shape

• Semen pH: Evaluation of acid–base balance

• Viscosity and Liquefaction Time: Assessment of semen consistency and fluidity

• White Blood Cells and Other Cells: Detection of infection or inflammation

• Anti-Sperm Antibodies: Assessed in selected cases to evaluate immune-related infertility

Spermogram Procedure

1. Sample Collection:
   Semen is usually collected by masturbation in a designated laboratory environment. In special cases, laboratory-approved condoms or electroejaculation may be used.

2. Laboratory Analysis:
   The sample is examined immediately after collection. The time between collection and analysis should not exceed 30 to 60 minutes.

3. WHO-Based Evaluation:
   All parameters are assessed according to the latest WHO guidelines.

Additional Tests Related to Spermogram

• Anti-sperm antibody test

• Sperm DNA Fragmentation (DFI) to assess sperm DNA integrity

• Semen biochemistry (Fructose and Zinc)

• Genetic testing and karyotyping

• Hormonal tests (FSH, LH, Testosterone)

When clinically indicated—such as in cases of low sperm motility, low sperm count, or a history of infertility—the laboratory also offers advanced tests including DNA Fragmentation Index (DFI) analysis.

Semen Sample Collection Instructions

Due to the sensitivity of this test and to ensure accurate results, please carefully follow the instructions below:

• A minimum of 48 hours (2 days) and a maximum of 7 days (168 hours) must have passed since the last ejaculation.

• The sample must be collected completely in the sterile container provided by the laboratory. Special attention should be paid to collecting the first portion of the sample.

• Avoid urination within 30 minutes prior to semen collection.

• Sample collection is performed according to the order of laboratory admission.

• Sample collection hours are daily from 12:30 PM to 2:00 PM.

• Avoid contamination of the semen sample with urine, soap, lubricants, or disinfectants.

• Inform laboratory staff if you have any chronic medical conditions (such as diabetes) or are taking any medications or substances, especially:

  • Antibiotics (e.g., erythromycin, gentamicin)

  • Cimetidine

  • Amiodarone

  • Digoxin

  • Phenytoin

  • Narcotics

  • Cigarettes

  • Hormonal medications

  • Chemotherapy drugs

  • Herbal medications

Why Is Spermogram Testing at Dr. Daneshbod Laboratory Different?

• Testing performed strictly according to WHO standards

• Expert interpretation by experienced pathologists

• Private and confidential sample collection environment

• Online access to test results

• Free consultation for result interpretation and guidance on further treatment steps

Importance of Spermogram in Infertility Treatment

Male infertility accounts for approximately 40–50% of infertility cases among couples. A spermogram provides detailed information about sperm count, motility, and quality, and can identify many fertility-related issues without the need for more complex tests.